Genomics, Transcriptomics and Proteomics

The course contents includes the following topics:

• Genome organisation of prokaryotes and eukaryotes.
• Mechanisms of genome stability, alteration and evolution, as well as Genomic recombination.
• Techniques of nucleic acids analysis.
• DNA markers technologies.
• Identification of genes only recognisable by their impact in the phenotype: a) Map based cloning; b) Genome wide association studies (GWAS); c) Identification of regions of homozygosity (ROHs).
• Genomic and expression libraries.
• Genomic databases.
• Next generation sequencing techniques.
• Quantitative analysis of gene expression, from microarrays to RNAseq, real time PCR and Epigenetic modulation.
• One genome multiple proteomes.
• Resolution of complex protein mixes through chromatographic techniques and 2D gel electrophoresis.
• Digestion techniques of proteins in peptides.
• Quantitative proteomics: Isotope (ICT) and Fluorescence (DIGE) labelling.
• Analysis of proteins by mass spectrometry.
• Identification of post-translational modifications.
• Protein arrays.

Final Competencies

1  Students are supposed to acquire theoretical knowledge and basic practical skills enabling them to feel more confident when joining modern companies involved in bio-production.

2  Ability to perform high-level modern scientific research in the field of Genomics, Transcriptomics and Proteomics.

3  Ability to initiate more advanced (e.g., Ph.D.) study programs that require relatively deep knowledge on the field of Genomics, Transcriptomics and Proteomics.

Further course information can be found here: https://studiekiezer.ugent.be/studiefiche/en/C004249/2021